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KMID : 0380620090410030302
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Korean Journal of Food Science and Technology
2009 Volume.41 No. 3 p.302 ~ p.306
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Quantification of Caffeic Acid and Rosmarinic Acid and AntioxidantActivities of Hot-water Extracts from Leaves of Perilla frutescens
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Lee Hyun-Sun
Lee Hyun-Ah
Hong Chung-Oui
Yang Sung-Yong
Hong Sung-Yu
Park Sang-Yul
Lee Ho-Jung
Lee Kwang-Won
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Abstract
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The principal objective of this investigation was to identify adequate species for the harvest of Perilla frutescensleaves, which provide profound antioxidant activities, and harbor abundant caffeic acid and rosmarinic acid. Namchun,Donggeul-2, Bora, Sae-bora and Neul-bora variants of the plant were assessed herein. In this study, we evaluated theantioxidant effects of these plants, and utilized an HPLC system to verify their caffeic acid and rosmarinic acid contents.Dried Perilla frutescens leaves were boiled at a temperature of 100oC for three hours, and were lyopholized in a freeze-dryer. The extracts were then processed in order to confirm their antioxidant activities via 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical-scavenging activity assay, ferric-reducing antioxidant power (FRAP) assay, total flavonoid content andtotal polyphenol content assays. According to the observed antioxidant results of the five tested species of Perillafrutescens leaves, Bora and Donggeul-2 were shown to have more potent antioxidant activities than the other testedvariants. The scavenge 50% of DPPH radical (SC50 of DPPH) values were 241μg DM/mL in Donggeul-2 and 261μgDM/mL in Bora. Based on the results of the FRAP assay, the Bora variant showed a value of 796mM FeSO4·7H2O/gDM, and Donggeul-2 exhibited a value of 748mM FeSO4·7H2O/g DM, of which the total polyphenol contents weremeasured as 69.4g GAE/kg DM and 61.8g GAE/kg DM, respectively. Moreover, the Bora variant had the highest levelof caffeic acid, and Donggeul-2 showed the highest rosmarinic acid content among the tested samples (0.87mg/100g wetbase and 121mg/100g wet base, respectively). According to the results of this experiment, we selected two species, Boraand Donggeul-2, which were both verified to contain adequate and favorable antioxidant activities.
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KEYWORD
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leaves of Perilla frutescens, antioxidant activities, caffeic acid, rosmarinic acid
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